Anti-RbAp46/RBBP7 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16576

Core Product Specifications and Parameters

Biological Background: RbAp46/RBBP7 Function and Localization

• RBBP7 (also known as RbAp46) is a core histone-binding protein that functions as a subunit within multiple chromatin-modifying complexes, including histone acetyltransferase (HAT), histone deacetylase (HDAC), nucleosome remodeling and deacetylase (NuRD), and Polycomb repressive complex 2 (PRC2/EED-EZH2).
• It plays a critical role in targeting chromatin remodeling enzymes to their histone substrates, a process regulated by nucleosomal DNA.
• This protein participates in key nuclear processes such as DNA replication-dependent chromatin assembly, transcriptional repression, and developmental gene regulation.
• The RBBP7 gene encodes a protein with a predicted molecular weight of approximately 48 kDa, containing multiple WD repeat domains that mediate protein-protein interactions.
• Subcellularly, RBBP7 is predominantly localized to the nucleus, consistent with its role in chromatin dynamics.
• Post-translational modifications of RBBP7, including acetylation, phosphorylation, and ubiquitin-like conjugation, modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-230) of RBBP7, likely generating antibodies that recognize both native and denatured forms; this supports broad application suitability.
• For Western blot (WB), consider using whole cell lysates from human, mouse, or rat cell lines, given proven cross-reactivity; the expected molecular weight is ~48 kDa—adjust gel percentage accordingly.
• In IHC-P and IF/ICC, validate antibody specificity with appropriate controls (e.g., blocking peptide or siRNA knockdown) and optimize antigen retrieval conditions.
• For ELISA, recombinant RBBP7 protein or lysates from RBBP7-expressing cells can serve as coating antigen, with careful titration of the detection antibody.
• Since RBBP7 is a component of multiple complexes, co-immunoprecipitation or pull-down assays may require condition optimization to preserve native interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, with deep expertise in chromatin biology and epigenetic targets. Beyond this RbAp46/RBBP7 monoclonal antibody, we offer validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to streamline your assay development. From concept to clinic, CamelBio bridges the gap between discovery and diagnostics, ensuring reliable supply for your chromatin and transcriptional regulation research.