Anti-RAV1 Rabbit Polyclonal Antibody for WB and ELISA - Q9ZWM9

Core Product Specifications and Parameters

Biological Background: RAV1 Function and Localization

• RAV1 is an AP2/ERF and B3 domain-containing transcription factor in Arabidopsis thaliana, also known as RELATED TO ABI3/VP1 1 and ETHYLENE RESPONSE DNA BINDING FACTOR 4.
• It specifically recognizes a bipartite DNA motif composed of 5'-CAACA-3' and 5'-CACCTG-3' sequences, acting as a transcriptional regulator.
• RAV1 functions as a negative regulator of plant growth and development, potentially modulating responses to ethylene and other developmental signals.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• It is broadly expressed across tissues, with highest levels in roots and rosette leaves, and very low expression in flowers.
• As a member of the RAV family, it shares structural features with ABI3/VP1-related proteins and integrates ethylene signaling pathways.
• Structural studies indicate defined DNA-binding domains that facilitate its specific interaction with target promoters.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider enriching nuclear extracts to improve detection sensitivity, as RAV1 is a nuclear protein.
• A band at approximately 39 kDa is expected; verify molecular weight using appropriate markers and consider peptide blocking experiments to confirm specificity of the polyclonal antibody.
• In ELISA, use recombinant RAV1 protein or synthetic peptide as a standard to validate reactivity and determine optimal antibody dilution.
• Cross-reactivity is confirmed for Arabidopsis thaliana; validation in other species or sample types should be performed as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing reliable access to research-grade antibodies like this anti-RAV1 polyclonal antibody for plant transcription factor studies. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials to support your diagnostic and research pipelines from concept to clinic.