Anti-Rat C-Reactive Protein/CRP Rabbit Polyclonal Antibody for WB, ELISA - P48199

Core Product Specifications and Parameters

Biological Background: C-reactive protein Function and Localization

• C-reactive protein (CRP) is encoded by the Crp (also known as Ptx1) gene in rat and functions as a key acute-phase reactant.
• It plays a central role in host defense by promoting agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine.
• CRP can also interact with DNA and histones, suggesting a role in scavenging nuclear material released from damaged circulating cells (By similarity).
• The protein is secreted and primarily found in plasma, where it participates in innate immune surveillance.
• It contains a calcium-binding site and belongs to the pentraxin family; activity is metal ion-dependent.
• Post-translational modifications include glycosylation and the presence of a disulfide bond.
• The precursor protein has a molecular weight of approximately 25.5 kDa (25468 Da according to UniProt), consistent with the observed band size.

Experimental Guidance and Technical Tips

• The immunogen was designed as a recombinant fusion protein encompassing amino acids 20–230 of rat CRP, which likely yields antibodies targeting multiple epitopes.
• For Western blot, a specific band around 25 kDa is anticipated; consider validating in the relevant sample system (e.g., rat plasma or liver tissue) before use.
• Performance in ELISA may require optimization of coating conditions and detection antibody pairing; polyclonal nature may contribute to sensitivity but should be empirically evaluated.
• As a polyclonal preparation, lot-to-lot consistency should be verified for quantitative applications.
• Cross-reactivity with other species or related pentraxins has not been tested; if needed, consider screening against the expected sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, providing validated raw materials from concept to clinic. This rat CRP polyclonal antibody is part of our expanding catalog supporting inflammation and cardiovascular disease research. Beyond individual antibodies, we offer matched antibody pairs, recombinant antigens, and bulk ancillary reagents, along with custom sourcing for rare or specialized targets—streamlining your entire assay development workflow.