Anti-RASSF6 Rabbit Polyclonal Antibody for WB, ELISA - Q6ZTQ3

Core Product Specifications and Parameters

Biological Background: RASSF6 Function and Localization

• RASSF6 (Ras association domain-containing protein 6) is a member of the RASSF family of tumor suppressors.
• It promotes apoptosis through both caspase-dependent and caspase-independent pathways, and may function as a Ras effector.
• RASSF6 may suppress serum-induced basal levels of NF-kappa-B, linking it to inflammatory and survival signaling.
• The protein is subject to alternative splicing, producing multiple isoforms, and is classified as a phosphoprotein.
• Highest expression is observed in thymus, kidney, and placenta, with lower levels in colon, small intestine, and lung.
• Expression is frequently down-regulated in 30–60% of tumors from breast, colon, kidney, liver, rectum, pancreas, stomach, and thyroid, suggesting a role in tumor suppression.
• The canonical isoform has a molecular weight of approximately 43 kDa (UniProt: 43,384 Da).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1–303 of human RASSF6 (NP_001257320.1), covering the full-length protein; consider this when setting up detection assays.
• For Western blot (WB), a band near 43 kDa is expected; validate specificity using control lysates from human, mouse, or rat tissues, as cross-reactivity is reported.
• For ELISA, the antibody may be used for direct or indirect detection; optimize coating antigen concentration and antibody dilution in your system.
• Since RASSF6 expression is regulated in tissues and tumors, include appropriate positive and negative controls (e.g., thymus vs. tumor samples) to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For researchers focusing on apoptosis, tumor suppression, or Ras signaling, we offer one-stop access to RASSF6 antibodies and related products, with expertise in sourcing rare targets and custom production to support your diagnostic workflow from concept to clinic.