Anti-RASSF5 Rabbit Polyclonal Antibody for WB and ELISA - Q8WWW0

Core Product Specifications and Parameters

Biological Background: RASSF5 Function and Localization

• RASSF5, also known as NORE1, NORE1A, NORE1B, RASSF3, Maxp1, or RAPL, is a potential tumor suppressor that is frequently down-regulated in lung tumor cell lines and primary lung tumors.
• Isoform 2 of RASSF5 plays a key role in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation, stimulating lymphocyte polarization and enhancing adhesion to ICAM1.
• In endothelial cells, the association of RASSF5 isoform 2 with activated RAP1A is required for directional movement during wound healing.
• RASSF5, together with RAP1A, may participate in the regulation of microtubule growth.
• The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS-induced apoptosis.
• RASSF5 localizes to the cytoplasm and associates with the cytoskeleton.
• The protein contains a zinc-finger domain, undergoes acetylation and phosphorylation, and is annotated with keywords including alternative splicing, apoptosis, and tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-265 amino acids of human RASSF5 (NP_872606.1). When using this antibody, consider that it may recognize multiple isoforms of RASSF5 (the region spans part of the protein), including the widely expressed isoform 1 and isoform 2. Validate specificity in your sample system.
• For Western blotting, the expected molecular weight of RASSF5 is approximately 47 kDa. Use appropriate loading controls to confirm band identity, as post-translational modifications may cause slight shifts.
• The antibody has been shown to cross-react with mouse samples; however, always verify cross-reactivity in your specific tissue or cell type, as expression levels may vary.
• For ELISA, optimize coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio, considering the polyclonal nature of the antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting for oncology and immunology research, including high-quality antibodies like this anti-RASSF5 polyclonal antibody. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic.