Anti-RASSF5 Polyclonal Antibody for WB, ELISA - Q8WWW0

Core Product Specifications and Parameters

Biological Background: RASSF5 Function and Localization

• RASSF5, also known as NORE1 and RAPL, is a potential tumor suppressor that undergoes alternative splicing, producing distinct isoforms with roles in apoptosis and cell adhesion.
• The protein functions as a Ras effector, mediating HRAS and KRAS-induced apoptosis through the RASSF5-STK4/MST1 complex.
• Isoform 2 links RAP1A activation upon TCR or chemokine stimulation to integrin activation, promoting lymphocyte polarization and adhesion to ICAM1.
• RASSF5 is involved in microtubule growth regulation and directional endothelial cell migration during wound healing, in association with activated RAP1A.
• Subcellularly, RASSF5 localizes to the cytoplasm and associates with the cytoskeleton, consistent with its role in cell adhesion and migration.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains a zinc-finger domain and metal-binding sites.
• Widely expressed across tissues, RASSF5 is frequently down-regulated in lung tumor cell lines and primary lung tumors, supporting its tumor suppressor role.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human, mouse, or rat cell lines; the immunogen spans the N-terminal 265 amino acids, targeting a region likely conserved across species, so adjust lysis and antibody dilution appropriately.
• The predicted molecular weight is approximately 47 kDa; always run suitable molecular weight markers and consider potential post-translational modifications that may shift the band.
• In ELISA, validate binding using recombinant RASSF5 protein or peptide; note that the polyclonal nature may produce strong signals but also potential cross-reactivity.
• Given the tumor suppressor context and downregulation in lung cancer, comparing normal versus tumor tissue lysates may provide informative results.
• Since RASSF5 localizes to the cytoplasm and cytoskeleton, subcellular fractionation or immunofluorescence may be considered if the antibody is validated for such applications, though only WB and ELISA are currently listed.
• Store the antibody as recommended and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This RASSF5 antibody supports research in tumor biology, cell adhesion, and apoptosis, and is part of CamelBio's broad portfolio of validated polyclonal and monoclonal antibodies. Explore our sourcing capabilities for antibody pairs, optimized reagents, and rare target raw materials to accelerate your diagnostic development.