Anti-RASSF4 Polyclonal Antibody for WB, ELISA - Q9H2L5

Core Product Specifications and Parameters

Biological Background: RASSF4 Function and Localization

• RASSF4 (Ras association domain-containing protein 4) is a potential tumor suppressor that may act as a downstream effector of the KRAS oncoprotein.
• It promotes apoptosis and induces cell cycle arrest, mechanisms key to its tumor-suppressive function.
• Widely expressed in normal tissues, RASSF4 is frequently down-regulated in tumor cell lines, suggesting its loss contributes to oncogenesis.
• The protein is subject to alternative splicing, resulting in multiple isoforms with potentially distinct functions.
• RASSF4 is a phosphoprotein, indicating regulation by phosphorylation signaling cascades.
• It has been identified in large-scale proteomics screens, supporting its broad expression and functional relevance.
• The encoded protein features a Ras association (RA) domain, common to this family, enabling interaction with RAS GTPases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-160) of human RASSF4. For WB, consider using a positive control such as human, mouse, or rat tissue lysates where RASSF4 is expressed.
• Due to the polyclonal nature, expect detection of multiple bands if alternative splicing variants are present. Validate band specificity using appropriate controls.
• For ELISA, the antibody can be used for direct or indirect detection; optimize coating and detection conditions with the recombinant immunogen as a reference standard.
• Since RASSF4 is frequently down-regulated in tumors, it may serve as a marker for tumor suppressor studies. Validate expression levels in cancer vs. normal cell lines.
• The antibody is reported to cross-react with mouse and rat; we recommend verifying reactivity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a streamlined supply of high-quality IVD raw materials. For RASSF4-targeted studies, we offer this validated polyclonal antibody alongside a comprehensive portfolio of apoptosis and tumor suppressor-related reagents. Our sourcing network also includes bulk ancillary reagents and rare target antibodies to accelerate your assay development.