Anti-RASIP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5U651

Core Product Specifications and Parameters

Biological Background: RASIP1 Function and Localization

• RASIP1 (Ras-interacting protein 1), also known as RAIN, is a 103 kDa protein encoded by the RASIP1 gene on human chromosome 19.
• It is required for proper vascular structure formation through both vasculogenesis and angiogenesis, acting as a critical, vascular‑specific regulator of GTPase signaling, cell architecture, and adhesion.
• RASIP1 modulates Rho GTPase activity by recruiting ARHGAP29, suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells, essential for endothelial morphogenesis and blood vessel tubulogenesis.
• It may serve as an effector for Golgi‑bound HRAS and other Ras‑like proteins, potentially promoting HRAS‑mediated transformation; it is also reported as a negative regulator of amino acid starvation‑induced autophagy.
• The protein localizes to the cytoplasm, perinuclear region, and Golgi apparatus, particularly the Golgi stack.
• Expression is highest in heart, with lower levels detected in placenta and pancreas, reflecting its role in cardiovascular and vascular biology.
• Key post‑translational modifications include methylation and phosphorylation (phosphoprotein), and it has been identified in global proteomics screens (reference proteome).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 635–785 at the C‑terminal region of human RASIP1; consider this when selecting detection or capture configurations in ELISA.
• For Western blot, a band around 103 kDa is expected; validate specificity in endothelial‑rich samples (e.g., HUVEC lysates) given the tissue‑specific expression profile.
• In immunofluorescence/ICC, anticipate perinuclear/Golgi staining; optimize permeabilization and fixation to preserve Golgi morphology.
• Cross‑reactivity with mouse and rat orthologs suggests epitope conservation; confirm reactivity in the relevant species and tissue context for pre‑clinical studies.
• For ELISA, the recombinant fragment immunogen can be used as a standard or coating antigen; pairing with a matched detection antibody may enable sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti‑RASIP1 rabbit polyclonal antibody, targeting a key angiogenic regulator, exemplifies our ability to supply optimized tools for cardiovascular and endothelial research. Let us streamline your sourcing from concept to clinic with tailored raw‑material solutions and expert technical support.