Anti-RASGRP1 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9Z1S3

Core Product Specifications and Parameters

Biological Background: RASGRP1 Function and Localization

• RASGRP1 (RAS guanyl-releasing protein 1), also known as CalDAG-GEFII (Calcium and DAG-regulated guanine nucleotide exchange factor II), is a Ras-specific guanine nucleotide exchange factor that activates Ras by catalyzing GDP/GTP exchange.
• It functions as a key downstream effector of T-cell and B-cell antigen receptors, coupling receptor activation to the Ras-ERK/MAP kinase cascade, thereby regulating lymphocyte development, homeostasis, and differentiation.
• RASGRP1 is essential for NK cell cytotoxicity and ITAM-dependent cytokine production, activating both ERK and JNK pathways.
• In mast cells, RASGRP1 regulates degranulation and cytokine secretion, controlling FcεRI-mediated allergic responses. Related references: PMID:17190838
• When deregulated, RASGRP1 acts as a proto-oncogene, promoting T-cell lymphomagenesis. Related references: PMID:15829980 PMID:17210708
• Subcellularly, RASGRP1 localizes to the cytoplasm, cytosol, and membrane compartments including the cell membrane, Golgi apparatus, and endoplasmic reticulum, consistent with its role in signal transduction.
• It is predominantly expressed in spleen and thymus, and detected at the protein level in mature thymocytes, bone marrow-derived mast cells, B-cells, and keratinocytes.
• Structurally, RASGRP1 contains a coiled-coil domain, zinc-finger motifs, and is regulated by calcium and DAG binding.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 678–795 in the C-terminal region of mouse RASGRP1; this region is conserved between mouse and human, supporting cross-reactivity.
• For Western blotting, a predominant band near 90 kDa may be observed; consider validating lysates from spleen, thymus, or immune cell lines.
• For immunoprecipitation, use standard protocol conditions; the polyclonal nature may enrich target effectively; verify with protein A/G agarose.
• In ELISA, use purified recombinant protein or cell lysates as coating antigen to assess binding.
• As a rabbit polyclonal antibody, it recognizes multiple epitopes; specificity should be confirmed in knockout or knockdown systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner, streamlining access to high-quality IVD raw materials for RASGRP1 and related targets. Beyond this polyclonal antibody, we supply validated antibody pairs, optimized monoclonal preparations, bulk ancillary reagents, and can source rare raw materials to meet your development needs—from early research to clinical application.