Anti-RASAL2 Rabbit Polyclonal Antibody for WB and ELISA - Q9UJF2

Core Product Specifications and Parameters

Biological Background: RASAL2 Function and Localization

• RASAL2, also known as nGAP and RAS protein activator-like 2, functions as an inhibitory regulator of the Ras-cyclic AMP pathway.
• As a member of the RAS GTPase-activating protein (GAP) family, it is predicted to negatively regulate Ras signaling by accelerating GTP hydrolysis.
• The gene undergoes alternative splicing, producing multiple protein isoforms (Keyword: Alternative splicing).
• RASAL2 is subject to phosphorylation, which may modulate its activity or interactions (Keyword: Phosphoprotein).
• It has been identified in proteomics studies and is part of the human reference proteome (Keywords: Proteomics identification, Reference proteome).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 750–1040, a region that may be conserved among isoforms; consider verifying isoform specificity using isoform-specific controls.
• For Western blot, a recommended starting dilution is 1:500 to 1:2000; optimize in the relevant sample system. Due to alternative splicing, multiple bands around 129 kDa may be observed.
• For ELISA, the antibody can be used as a capture or detection reagent; validate with recombinant RASAL2 or cell lysates expressing endogenous protein.
• Validate antibody performance under your specific experimental conditions, including appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. With expertise in rare target raw-material procurement, we support research on RAS signaling pathways by providing high-quality anti-RASAL2 antibodies and tailored technical consultation. Whether you need small-scale research aliquots or large-scale manufacturing batches, our integrated supply chain ensures consistent, traceable immunoreagents for every stage from concept to clinic.