Anti-RARS2 Polyclonal Antibody for WB, ELISA - Q5T160

Core Product Specifications and Parameters

Biological Background: RARS2 Function and Localization

• RARS2 encodes the probable mitochondrial arginine-tRNA ligase, also known as arginyl-tRNA synthetase (ArgRS), a member of the class-I aminoacyl-tRNA synthetase family.
• It catalyzes the ATP-dependent attachment of arginine to its cognate tRNA(Arg) in a two-step reaction, essential for mitochondrial protein translation.
• The protein localizes to the mitochondrial membrane, consistent with its role in mitochondrial translation.
• Mutations in RARS2 are associated with pontocerebellar hypoplasia type 6 (PCH6), a severe neurodegenerative disorder.
• The enzyme features ATP-binding and nucleotide-binding domains characteristic of ligases, and has a predicted molecular weight of approximately 66 kDa.
• Post-translational modifications include acetylation, which may regulate its function or stability.
• It plays a critical role in mitochondrial protein biosynthesis, supporting oxidative phosphorylation and cellular energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans a large fragment (aa 17-340) of human RARS2, which should enable recognition of the full-length protein in human and mouse samples.
• For Western blot, a band around 66 kDa (predicted) may be observed; consider using mitochondrial-enriched lysates to enhance signal detection.
• Due to high sequence conservation, cross-reactivity between human and mouse is expected; validate independently in each species of interest.
• ELISA can be used for quantitative detection; optimize conditions by coating with recombinant RARS2 protein or cell lysates.
• Since RARS2 is mitochondrial, sample preparation with mild detergents may improve extraction efficiency.
• As a polyclonal antibody, lot-to-lot variability may occur; always titrate and include appropriate controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. For studies involving mitochondrial aminoacyl-tRNA synthetases and related disorders, CamelBio offers validated antibodies, recombinant proteins, and ancillary reagents to support assay development and translational research. Whether your project requires rare-target antibodies or bulk quantities of core raw materials, CamelBio serves as your reliable sourcing bridge from concept to clinic.