Anti-RARS Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P54136

Core Product Specifications and Parameters

Biological Background: RARS Function and Localization

• RARS is the cytoplasmic arginyl-tRNA synthetase (ArgRS), encoded by the RARS1 gene.
• It is a key component of the multi-tRNA synthetase complex, catalyzing the attachment of arginine to its cognate tRNA for protein synthesis (PubMed:25288775). Related references: PMID:25288775
• Beyond translation, RARS modulates AIMP1 secretion and may be involved in the generation of the inflammatory cytokine EMAP2 (PubMed:17443684). Related references: PMID:17443684
• The protein is predominantly located in the cytoplasm and cytosol.
• RARS undergoes post-translational modifications including acetylation and alternative initiation.
• Mutations in RARS are associated with leukodystrophy, highlighting its importance in neural health.
• Keywords: Aminoacyl-tRNA synthetase, ATP-binding, Ligase, Nucleotide-binding, Protein biosynthesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-300) of human RARS; consider that detection may be specific to isoforms containing this domain.
• For Western blotting under reducing conditions, a band around 75 kDa is expected based on the predicted molecular weight.
• Validate antibody performance in IHC-P and IF/ICC by optimizing fixation and permeabilization conditions for your sample type.
• For ELISA applications, confirm optimal coating and detection conditions using the recombinant protein or endogenous target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For research on protein synthesis and aminoacyl-tRNA synthetases, this anti-RARS antibody can be integrated into your diagnostic workflows. Our one-stop sourcing capabilities extend to rare targets and custom reagent development, supporting your projects from concept to clinic.