Anti-RARRES2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99969

Core Product Specifications and Parameters

Biological Background: RARRES2 Function and Localization

• RARRES2 (chemerin) is a secreted adipokine encoded by the RARRES2 gene, also known as TIG2 or tazarotene-induced gene 2 protein.
• It regulates adipogenesis, metabolism, and inflammation through activation of the chemokine-like receptor 1 (CMKLR1), and also acts as a ligand for CMKLR2. It can additionally bind CCRL2 with lower affinity. Related references: PMID:27716822
• Positively regulates adipocyte differentiation and modulates the expression of genes involved in lipid and glucose metabolism; may play a role in angiogenesis during white adipose tissue expansion.
• Exhibits dual pro- and anti-inflammatory properties depending on proteolytic cleavage; acts as a chemotactic factor for dendritic cells, macrophages, and NK cells.
• In vascular endothelial cells, exerts anti-inflammatory effects by preventing TNF-induced VCAM1 expression and monocyte adhesion via AKT1/NOS3 signaling and nitric oxide production.
• Highly expressed in placenta, liver, white adipose tissue (enriched in adipocytes), and skin (epidermis, hair follicles, endothelial cells).
• Expression elevated in metabolic and inflammatory diseases such as psoriasis, obesity, type 2 diabetes, metabolic syndrome, and cardiovascular disease.
• Displays antimicrobial activity in skin; contains a signal peptide and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 17-163 of human RARRES2, covering nearly the full-length secreted protein. For Western blot, a band may be observed near 19 kDa.
• For immunofluorescence/immunocytochemistry, consider that RARRES2 is a secreted protein; intracellular staining may require permeabilization and stimulation of release.
• Validate antibody performance in appropriate positive control samples (e.g., human adipose tissue or liver lysates) and consider species cross-reactivity with mouse samples as indicated.
• When performing ELISA, pair this polyclonal antibody with a suitable detection antibody; optimize conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research manufacturers by providing high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For adipokine and metabolic disease research, we offer one-stop sourcing of rare target antibodies and recombinant proteins, helping streamline your assay development from concept to clinic.