Anti-RARG Rabbit Polyclonal Antibody for WB, ELISA - P13631

Core Product Specifications and Parameters

Biological Background: Retinoic acid receptor gamma Function and Localization

• Encoded by the RARG gene (also known as NR1B3), retinoic acid receptor gamma (RAR-gamma) belongs to the nuclear receptor superfamily and functions as a ligand-activated transcription factor.
• Binds as a heterodimer with retinoid X receptors (RXR) to retinoic acid response elements (RAREs) composed of tandem AGGTCA motifs, regulating gene expression in response to all-trans and 9-cis retinoic acid.
• Acts predominantly as a transcriptional activator even in the absence of ligand due to weak corepressor binding, distinguishing it from other RAR subtypes.
• Plays essential roles in embryonic development, particularly in limb bud formation, and cooperates with RAR-alpha and RAR-beta in skeletal growth, matrix homeostasis, and growth plate function.
• Localizes to both the nucleus and cytoplasm, enabling signal-dependent gene regulation and potential non-genomic functions.
• Undergoes post-translational modifications including sumoylation (Ubl conjugation), methylation, and isopeptide bond formation, which may modulate stability and activity.
• Expressed at the protein level in aortic endothelial cells, suggesting a role in vascular biology.
• Keywords: Alternative splicing, DNA-binding, Metal-binding, Zinc-finger; indicating structural features critical for DNA recognition and receptor function.

Experimental Guidance and Technical Tips

• The immunogen (aa 168-199) lies within a region unique to RARG among RAR subtypes; this antibody may exhibit minimal cross-reactivity with RARA or RARB. Validate specificity in knockout models if available.
• For Western blotting, a band around 50 kDa is expected; however, post-translational modifications may cause slight shifts. Optimize blocking and antibody dilution using lysates from RARG-expressing tissues or cell lines.
• In ELISA applications, this antibody can be considered for both direct and sandwich formats. Pair with a suitable detection antibody and validate using recombinant RARG protein or cell extracts.
• The antibody cross-reacts with mouse and rat RARG, enabling comparative studies. Confirm performance in each species' sample type due to potential differences in protein abundance or modification.
• Storage upon arrival: since shipping is on ice bags, aliquot and store at -20°C or -80°C to maintain stability. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD developers, offering a wide range of raw materials including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized custom sourcing for rare targets. This anti-RARG polyclonal antibody supports research in retinoic acid signaling, transcriptional regulation, and developmental biology, with applications in ELISA and Western blotting. From discovery to clinical assay development, CamelBio's supply chain expertise and technical support help streamline your workflow.