Anti-RARG Rabbit Monoclonal Antibody for WB, ELISA - P13631

Core Product Specifications and Parameters

Biological Background: RARG Function and Localization

• Retinoic acid receptor gamma (RARG), also designated NR1B3 or RARgamma, is a ligand-inducible transcription factor belonging to the nuclear receptor superfamily.
• RARG heterodimerizes with retinoid X receptors (RXRs) and binds to retinoic acid response elements (RAREs) consisting of direct repeats of the hexameric motif 5'-AGGTCA-3'.
• In the absence of ligand, RARG can act as a constitutive activator of transcription due to inefficient corepressor recruitment; upon binding all-trans or 9-cis retinoic acid, it undergoes a conformational change to regulate target gene expression.
• Among its developmental functions, RARG is essential for limb bud development and, together with RARA and RARB, promotes skeletal growth, matrix homeostasis, and growth plate maintenance.
• At the protein level, RARG is detected in aortic endothelial cells, indicating a role in vascular biology.
• The receptor is primarily localized in the nucleus but can also be found in the cytoplasm, consistent with its function as a DNA-binding transcription factor.
• RARG undergoes multiple post-translational modifications, including phosphorylation, methylation, and ubiquitination (via isopeptide bonds), which fine-tune its stability and transcriptional activity.
• Alternative splicing produces several RARG isoforms that may have distinct tissue distributions and functional properties.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide spanning the N-terminal region (amino acids 1–100) of human RARG, a domain that is relatively conserved across human, mouse, and rat.
• For Western blot applications, nuclear extracts or whole-cell lysates from retinoic acid‑responsive cell lines (e.g., MCF-7, HL-60) may provide a suitable positive control; under reducing conditions, the antibody is expected to detect a band around 50 kDa.
• In ELISA, consider using the antibody for solid‑phase immunoassays after confirming specificity against the target peptide or recombinant RARG protein.
• It is advisable to optimize antibody concentration and assay conditions for each experimental system, as performance can vary with sample preparation and expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers and researchers with reliable antibodies, antigens, and ancillary reagents. This anti-RARG monoclonal antibody is part of our offering for nuclear receptor and developmental biology research. We also support custom procurement for rare targets and bulk‑volume needs, streamlining your pathway from concept to clinic.