Anti-Raptor Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8N122

Core Product Specifications and Parameters

Biological Background: Raptor Function and Localization

• Raptor (regulatory-associated protein of mTOR) is a key component of mTOR complex 1 (mTORC1), a central nutrient sensor that promotes anabolic processes including protein, lipid, and nucleotide synthesis while inhibiting catabolic pathways such as autophagy (PubMed:12150925, PubMed:12150926, PubMed:12747827). Related references: PMID:12150925 PMID:12150926 PMID:12747827
• Raptor acts as a dual adapter within mTORC1: it recruits mTORC1 to lysosomal membranes by interacting with Rag GTPases, and it binds TOS motif-containing substrates to direct them for phosphorylation by mTOR kinase (PubMed:12747827, PubMed:24403073, PubMed:26588989). Related references: PMID:12747827 PMID:24403073 PMID:26588989
• mTORC1 phosphorylates downstream effectors such as S6K1 and 4E-BP1 to drive ribosome biogenesis and translation initiation; it also phosphorylates ULK1 and TFEB to suppress autophagy (PubMed:12150925, PubMed:12150926, PubMed:12747827). Related references: PMID:12150925 PMID:12150926 PMID:12747827
• The protein localizes primarily to the lysosome membrane, cytoplasm, and cytoplasmic granules, consistent with its role in nutrient sensing and signaling.
• Raptor undergoes multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, and is predicted to be a glycoprotein; it also contains WD40 repeats important for protein-protein interactions.
• Tissue expression is highest in skeletal muscle, with moderate levels in brain, lung, small intestine, kidney, and placenta; it is widely expressed with highest levels in nasal mucosa and pituitary.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (amino acids 1236–1335) of human Raptor, a region conserved in mouse, supporting the reported cross-reactivity.
• For Western blotting, the expected band is approximately 149 kDa; a suitable molecular weight marker and appropriate gel percentage should be selected.
• Antigen retrieval and optimal antibody dilution are recommended for immunohistochemistry (IHC-P); validate staining patterns in appropriate human or mouse tissue controls.
• For ELISA applications, consider direct or sandwich formats depending on the detection system; confirm sensitivity and specificity with recombinant antigen or cell lysates.
• This antibody is provided as a rabbit monoclonal, ensuring high lot-to-lot reproducibility and specificity for routine assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to validated antibodies, protein reagents, and customized sourcing for mTOR pathway targets like Raptor. Whether you need bulk monoclonal antibodies, optimized antibody pairs, or ancillary reagents, our team streamlines procurement from concept to clinic. Partner with CamelBio to accelerate your assay development with high-quality raw materials and expert support.