Anti-RAPSN Polyclonal Antibody for WB, IHC-P, ELISA - Q13702

Core Product Specifications and Parameters

Biological Background: RAPSN Function and Localization

• RAPSN encodes a 43 kDa postsynaptic protein, also known as 43 kDa receptor-associated protein of the synapse or RING finger protein 205. Multiple isoforms exist via alternative splicing.
• The protein functions as a critical scaffold at the neuromuscular junction, essential for clustering nicotinic acetylcholine receptors (nAChRs) on the postsynaptic membrane.
• It may link nAChRs to the cytoskeleton via direct interaction with actin or spectrin, stabilizing the postsynaptic architecture.
• RAPSN belongs to the RING finger protein family, containing zinc-finger and TPR repeat domains, which mediate protein-protein interactions.
• Subcellular localization includes the postsynaptic cell membrane and cytoplasm, associated with the cytoskeleton.
• Mutations in RAPSN are associated with congenital myasthenic syndrome (CMS), underscoring its essential role in neuromuscular transmission.
• Post-translational modifications include myristoylation, phosphorylation, and ubiquitylation, which may regulate its function and localization.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of RAPSN is ~46 kDa. Use standard SDS-PAGE and transfer conditions, and validate the antibody with a positive control lysate from human, mouse, or rat muscle tissue.
• For immunohistochemistry (IHC-P), antigen retrieval may be required. Optimize fixation and permeabilization steps for your sample type, and consider using an appropriate blocking buffer to reduce background.
• For ELISA, the antibody can be used in a direct or indirect assay format. Pair it with a suitable recombinant RAPSN protein or peptide to ensure specificity.
• The antibody is a polyclonal raised in rabbit; therefore, secondary antibodies should be anti-rabbit and compatible with your detection system. Minimize cross-reactivity by using species-adsorbed secondary antibodies if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between concept and clinic by providing diagnostic manufacturers and researchers with one-stop access to a vast portfolio of IVD raw materials, technical services, and consulting. Our offerings include validated antibody pairs, monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents, all supported by robust supply chains. As a sourcing partner for neuromuscular junction and autoimmune disease research, we ensure seamless procurement of high-quality reagents like the anti-RAPSN antibody, empowering your diagnostic development from early-stage discovery to clinical application.