Anti-RAPH1 Rabbit pAb for WB, IF/ICC, ELISA - Q70E73

Core Product Specifications and Parameters

Biological Background: RAPH1 Function and Localization

• RAPH1 (Ras-associated and pleckstrin homology domains-containing protein 1), also known as Lamellipodin, PREL-2, and ALS2CR18/ALS2CR9, contains PH and RA domains typical of signaling adaptors that link membrane signals to the cytoskeleton.
• Core function: Acts as a mediator of localized membrane signals, critically regulating lamellipodial dynamics and negatively regulating cell adhesion, thereby influencing cell motility and morphology.
• Gene family and isoforms: Encoded by the RAPH1 gene; alternative splicing produces multiple isoforms, including RMO1 and RMO1-RAPH1, which display distinct tissue-specific expression patterns.
• Subcellular localization: Found at the cell membrane and on the cytoskeleton, with enrichment in lamellipodia and filopodia—dynamic actin-based protrusions essential for cell migration and adhesion.
• Tissue distribution: Ubiquitously expressed across tissues; isoform RMO1-RAPH1 shows highest levels in brain, heart, ovary, and developing embryo, while isoform RMO1 is most abundant in liver and low in B-cells.
• Post‑translational modifications: Subject to acetylation and phosphorylation; categorized as a phosphoprotein, and its 3D structure has been experimentally determined, underscoring its role in signaling and cytoskeletal remodeling.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant fusion protein spanning the N‑terminal region (aa 1‑240) of human RAPH1; consider verifying isoform detection specificity using knockdown or isoform‑specific transcript analysis.
• For Western blotting, the predicted molecular weight is ~135 kDa; ensure adequate protein extraction from fresh or well‑preserved samples and consider adding phosphatase inhibitors to preserve phosphorylation states.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization is recommended to access membrane‑associated and cytoskeletal pools; perform appropriate controls to distinguish specific from nonspecific signals.
• Cross‑reactivity with mouse and rat has been reported; when using these species, confirm sequence homology in the immunogen region and optimize conditions for each model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner for diagnostic manufacturers, labs, and research institutes, providing seamless access to validated antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-RAPH1 polyclonal antibody exemplifies our focus on cell signaling and cytoskeletal research tools, and we can supply matched antibody pairs, optimized monoclonal/polyclonal antibodies, and technical consultation to accelerate your assay development from concept to clinic.