Anti-RAPGEF1 (C3G/GRF2) Polyclonal Antibody for WB, IF/ICC, ELISA - Q13905

Core Product Specifications and Parameters

Biological Background: RAPGEF1 Function and Localization

• RAPGEF1 (also known as C3G, GRF2) is a guanine-nucleotide-releasing protein that binds to the SH3 domain of CRK and GRB2/ASH, transducing signals from CRK to activate RAS.
• It is involved in cell branching and adhesion through the BCAR1-CRK-RAPGEF1 signaling cascade, leading to activation of RAP1. Related references: PMID:12432078
• Plays a role in the establishment of basal endothelial barrier function, contributing to vascular integrity.
• Mediates nerve growth factor (NGF)-induced sustained activation of Rap1 and promotes neurite outgrowth, highlighting its importance in neurogenesis.
• The protein localizes primarily to early endosomes, suggesting involvement in endosomal signaling.
• RAPGEF1 is ubiquitously expressed in adult and fetal tissues, with high expression in adult skeletal muscle and placenta, and in fetal brain and heart; expression is low in adult and fetal liver.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond); alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment encompassing amino acids 844-1095, and may detect multiple RAPGEF1 isoforms (theoretical MW ~121 kDa).
• For Western blotting (WB), a strong band around 121 kDa is typically observed; confirm specificity using appropriate positive and negative controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimization of fixation and permeabilization conditions is recommended to preserve early endosomal localization.
• For ELISA applications, consider standard antibody titration and antigen coating conditions; cross-reactivity with mouse and rat samples may allow flexible experimental designs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-RAPGEF1 antibody supports research in cell adhesion, RAS activation, and neurobiology, and we can assist with custom sourcing and technical consulting to accelerate your project from concept to clinic.