Anti-Rap1GAP Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P47736

Core Product Specifications and Parameters

Biological Background: Rap1GAP Function and Localization

• Rap1GAP, officially designated Rap1 GTPase-activating protein 1, is encoded by the RAP1GAP gene (also known as KIAA0474 and RAP1GA1).
• It functions as a GTPase activator for RAP-1A, accelerating the hydrolysis of GTP to GDP and thereby converting RAP-1A to its inactive state.
• The protein localizes predominantly to the Golgi apparatus membrane, implicating it in membrane-associated signaling and trafficking.
• Tissue expression is highest in the brain (especially cerebral cortex), kidney, and pancreas, while being notably absent from lymphoid tissues.
• Classified as a phosphoprotein, Rap1GAP is subject to phosphorylation events that may regulate its activity or interactions.
• Alternative splicing of the RAP1GAP gene generates multiple transcript variants, increasing functional diversity.
• With a calculated molecular mass of ~73 kDa (73,361 Da), the protein is suitable for western blot detection at the expected weight.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1–100) of human Rap1GAP. When designing immunoassays, consider that the epitope may be localized to this domain; verify accessibility in native protein conformations and across splice variants.
• For western blotting, Rap1GAP migrates at ~73 kDa; validate detection in lysates from tissues with high expression such as brain or kidney.
• In immunofluorescence/immunocytochemistry, the Golgi membrane localization suggests staining patterns should appear perinuclear and punctate; use appropriate organelle markers for co-localization studies.
• For ELISA applications, pair with a capture antibody targeting a distinct non-overlapping epitope if developing a sandwich assay.
• Given reported cross-reactivity with mouse and rat, the antibody is suitable for translational studies in rodent models; however, confirm specificity via knockdown or knockout controls in the chosen system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of critical raw materials for diagnostic developers by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This Rap1GAP monoclonal antibody supports research on GTPase signaling and intracellular trafficking, complementing our catalog of well-characterized reagents for neuroscience and oncology applications. Leverage our end-to-end supply chain expertise to accelerate your assay development from concept to clinic.