Anti-RAP1B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P61224

Core Product Specifications and Parameters

Biological Background: Ras-related protein Rap-1b Function and Localization

• RAP1B is a member of the Ras superfamily of small GTPases, functioning as a molecular switch by cycling between GDP-bound inactive and GTP-bound active states.
• It possesses intrinsic GTPase activity and contributes to the establishment and maintenance of endothelial cell polarity and vascular lumen integrity.
• RAP1B is required for the proper localization of polarity proteins PRKCZ, PARD3, and TIAM1 to cell junctions, thereby supporting basal endothelial barrier function.
• The protein is found at the cell membrane, in the cytosol, and at cell junctions, reflecting its dynamic role in signal transduction.
• RAP1B undergoes post-translational modifications including prenylation and methylation, which are critical for membrane association and function.
• Alternative splicing results in multiple transcript variants, and the protein is subject to ADP-ribosylation and phosphorylation, as noted in UniProt keywords.
• It has been identified in various proteomics studies and is associated with disease variants, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 81–181 of human RAP1B (NP_056461.1), an internal region conserved across species; consider this when designing epitope-specific assays.
• For Western blot (WB), multiple bands (15–20 kDa) may be observed due to alternative splicing and post-translational modifications; validate with appropriate controls.
• In immunofluorescence (IF/ICC), RAP1B localizes to the cell membrane, cytoplasm, and cell junctions; optimize fixation and permeabilization to preserve these structures.
• For ELISA, confirm coating conditions and antibody specificity using recombinant RAP1B protein or relevant cell lysates.
• Cross-reactivity with mouse and rat is expected; however, always validate in the target sample system to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials. This RAP1B polyclonal antibody supports vascular biology and signal transduction research, and we offer validated antibody pairs, bulk reagents, and custom sourcing for rare targets. Partner with CamelBio to accelerate your assay development from concept to clinic with reliable, cost-effective raw-material solutions.