Anti-Rap1B Polyclonal Antibody for WB, IF/ICC, ELISA - P61224

Core Product Specifications and Parameters

Biological Background: Rap1B Function and Localization

• Rap1B (Ras-related protein Rap-1b), also known as GTP-binding protein smg p21B, is a member of the Ras small GTPase superfamily.
• Encoded by the RAP1B gene (Gene ID: 5908) in humans, the protein exists in multiple isoforms due to alternative splicing.
• Rap1B possesses intrinsic GTPase activity and functions as a molecular switch in signal transduction, cycling between active GTP-bound and inactive GDP-bound states.
• It plays a critical role in endothelial cell polarity and vascular lumen formation by contributing to the polarizing activity of KRIT1 and CDH5.
• Required for the localization of phosphorylated PRKCZ, PARD3, and TIAM1 to cell junctions, thereby establishing basal endothelial barrier function.
• Subcellularly, Rap1B localizes to the cell membrane, cytosol, and cell junctions.
• The protein undergoes several post-translational modifications including prenylation (lipid modification), phosphorylation, and methylation, which regulate its membrane association and activity.
• Clinically, RAP1B mutations are associated with disease variants, highlighting its importance in human health.

Experimental Guidance and Technical Tips

• For Western blot, use human or mouse cell lines known to express Rap1B (e.g., endothelial cells) as positive controls; a band at approximately 21 kDa is expected.
• In immunofluorescence/ICC, consider optimizing fixation and permeabilization conditions to preserve antigen accessibility, as Rap1B localizes to cell membrane and junctions.
• For ELISA, the antibody may be tested against recombinant Rap1B protein (such as the immunogen) to confirm binding; titrate the antibody for optimal signal-to-noise ratio.
• Given the polyclonal nature, the antibody may detect multiple Rap1B isoforms or related Rap family members; validate specificity in the relevant sample system.
• Cross-reactivity is reported for human and mouse; if using rat samples, conduct pilot experiments to verify reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your Rap1B-targeted studies in vascular biology and signal transduction, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-Rap1B pAb, and bulk ancillary reagents to support kit development. Count on CamelBio as your sourcing bridge for reliable, high-quality raw materials.