Anti-Rap1A Polyclonal Antibody for WB, IHC-P, ELISA - P62834

Core Product Specifications and Parameters

Biological Background: Rap1A Function and Localization

• Rap1A, or Ras-related protein 1A, is a small GTPase of the Ras superfamily, also known as C21KG, G-22K, GTP-binding protein smg p21A, and Krev-1.
• It counteracts the mitogenic function of Ras by competitively interacting with Ras GAPs and RAF. Related references: PMID:17916086
• Together with ITGB1BP1, Rap1A regulates KRIT1 localization to microtubules and membranes. Related references: PMID:17916086
• Plays a role in nerve growth factor (NGF)-induced neurite outgrowth.
• Involved in the regulation of embryonic blood vessel formation and establishment of basal endothelial barrier function.
• Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3. Related references: PMID:25208567
• Subcellular localization includes the cell membrane, cytoplasm (perinuclear region), cell junctions, and early endosomes.
• Key features: lipoprotein, prenylated, methylated, GTP-binding, and hydrolase activity; classified as a tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen targets a sequence within amino acids 39–138, covering a portion of the conserved GTPase domain. Consider performing a BLAST alignment to assess potential cross-reactivity with other Ras family members.
• For Western blot, a band around 21 kDa is expected; include appropriate molecular weight markers and validate using knockout or knockdown samples where possible.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and confirm specificity using tissue sections from knockout animals if available.
• The antibody has been validated for knockout in WB; however, performance in other applications should be evaluated in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined one-stop sourcing platform for IVD raw materials, supporting diagnostic developers from concept to clinic. This anti‑Rap1A polyclonal antibody is part of a broader portfolio covering cancer signaling, vascular biology, and neurogenesis targets. Beyond single antibodies, we supply validated antibody pairs, bulk recombinant proteins, and ancillary reagents, and can assist with sourcing rare or custom targets to accelerate your assay development.