Anti-RanGAP1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P46060

Core Product Specifications and Parameters

Biological Background: RanGAP1 Function and Localization

• RanGAP1, also known as SD, Fug1, or RANGAP, is encoded by the RANGAP1 gene (KIAA1835) and functions as a Ran GTPase-activating protein.
• It converts cytoplasmic GTP-bound Ran to GDP-bound Ran, essential for Ran-mediated nuclear import and export (PubMed:27160050, PubMed:8896452). Related references: PMID:27160050 PMID:8896452
• RanGAP1 mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN, and RANBP2 after nuclear export (PubMed:27160050). Related references: PMID:27160050
• It localizes to multiple subcellular compartments including the cytoplasm, nucleoplasm, nuclear envelope, centromere, kinetochore, and mitotic spindle, reflecting its roles in interphase transport and mitosis.
• RanGAP1 is highly expressed in brain, thymus, and testis, suggesting critical functions in these tissues.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitin-like conjugation (SUMOylation) regulate its activity and localization; the protein also contains leucine-rich repeats.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (aa 1-100) of human RanGAP1; this region may be conserved and enable detection of both full-length and N-terminal fragments.
• For Western blot, expect a band near 64 kDa in human, mouse, and rat samples; consider using nuclear or cytoplasmic extracts to match RanGAP1's dual localization.
• In IHC-P, antigen retrieval optimization is recommended; tissues with high RanGAP1 expression (e.g., brain, thymus, testis) can serve as positive controls.
• For IF/ICC, RanGAP1 displays nuclear envelope and spindle localization; live-cell or staged samples may reveal cell cycle-dependent dynamics.
• ELISA applications should be developed using appropriate coating conditions and validated with recombinant RanGAP1 standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research labs with a streamlined path to high-quality raw materials, from discovery antibodies to bulk reagents. For RanGAP1 and nuclear transport targets, we supply validated monoclonal antibodies, antigen standards, and custom sourcing to support assay development. Let CamelBio be your integrated partner in accelerating diagnostic innovation from concept to clinical application.