Anti-RanBP9 Rabbit Polyclonal Antibody for WB, ELISA - Q96S59

Core Product Specifications and Parameters

Biological Background: RanBP9 Function and Localization

• RanBP9 (Ran-binding protein 9, also known as RanBPM, BPM-L, BPM90, RanBP7) is a scaffolding protein that may act as an adaptor to couple membrane receptors to intracellular signaling pathways.
• It is a core component of the CTLH E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins such as HBP1. Related references: PMID:29911972
• RanBP9 enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway, and may be involved in integrin signaling, including ITGB2/LFA-1. Related references: PMID:12147692 PMID:14722085
• It modulates nuclear receptor activity: enhances dihydrotestosterone-induced transactivation of AR and dexamethasone-induced transactivation of NR3C1, but does not affect estrogen-induced transactivation. Related references: PMID:12361945 PMID:18222118
• RanBP9 stabilizes TP73 isoform Alpha, likely by inhibiting its ubiquitination, thereby increasing its proapoptotic activity. Related references: PMID:15558019
• Acts as a mediator of cell spreading and actin cytoskeleton rearrangement. Related references: PMID:18710924
• Subcellularly, RanBP9 is found in the cytoplasm, nucleus, and cell membrane. It is ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest in lung; within the brain, it is predominantly expressed in neurons of the cerebral cortex, cerebellar granular layer, and Purkinje cells.
• Additional features include post-translational modifications such as acetylation, phosphorylation, and ubiquitination, as well as alternative splicing and proteomics identification.

Experimental Guidance and Technical Tips

• For Western blot (WB), this polyclonal antibody, raised against a recombinant immunogen spanning amino acids 480–605, may recognize linear epitopes. A band of approximately 78 kDa is expected in human cell lysates; consider using a positive control such as HeLa or HEK293 whole cell extract.
• Due to potential isoform variations from alternative splicing, confirm the molecular weight and specificity in your target tissue or cell line.
• For ELISA, the antibody can be considered for direct detection of recombinant or native RanBP9; however, optimal coating concentration and detection conditions should be empirically determined.
• As cross-reactivity is reported for human, validate performance in other species of interest before use.
• The immunogen region corresponds to the C-terminal portion of the protein; note that some isoforms may lack this region, so verify antibody recognition if studying specific splice variants.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-RanBP9 rabbit polyclonal antibody as part of a comprehensive portfolio of IVD raw materials, providing diagnostic manufacturers and researchers with reliable reagents for studying scaffolding proteins, ubiquitin ligase complexes, and cell signaling pathways. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support assay development, as well as rare target sourcing capabilities to accelerate your diagnostic workflow from concept to clinic.