Anti-RanBP9 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q96S59

Core Product Specifications and Parameters

Biological Background: RanBP9 Function and Localization

• RanBP9 (Ran-binding protein 9), also known as BPM-L, BPM90, RanBPM, and RanBP7, is a ubiquitously expressed scaffolding/adapter protein encoded by the RANBP9 gene (Gene ID: 10048).
• RanBP9 is a core component of the CTLH E3 ubiquitin-protein ligase complex that mediates ubiquitination and proteasomal degradation of transcription factor HBP1. Related references: PMID:29911972
• It acts as a mediator of cell spreading and actin cytoskeleton rearrangement, and is involved in integrin signaling, including ITGB2/LFA-1. Related references: PMID:18710924 PMID:14722085
• RanBP9 enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Related references: PMID:12147692
• It modulates steroid hormone receptor transactivation, enhancing dihydrotestosterone-induced AR activity and dexamethasone-induced NR3C1 activity. Related references: PMID:12361945 PMID:18222118
• RanBP9 stabilizes TP73 isoform Alpha, inhibiting its ubiquitination and increasing proapoptotic activity. Related references: PMID:15558019
• It inhibits the kinase activity of DYRK1A and DYRK1B, and inhibits FMR1 binding to RNA.
• Subcellularly, RanBP9 localizes to the cytoplasm, nucleus, and cell membrane. Post-translational modifications include phosphorylation, acetylation, and ubiquitin-like conjugation.
• Tissue specificity: Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle; within the brain, expressed in neurons of the cortical gray matter, cerebellar granular layer, and Purkinje cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 100–200 of human RanBP9; consider sequence alignment with mouse and rat orthologs to confirm expected cross-reactivity.
• For Western blot, a band at ~78 kDa is anticipated; validate detection in your sample system with appropriate positive and negative controls.
• For IHC-P, antigen retrieval and antibody titration may require optimization; consider testing on known positive tissues such as testis or placenta where RanBP9 is highly expressed.
• In ELISA, the antibody may be used as a capture or detection reagent; confirm linearity and sensitivity with recombinant protein standards.
• Cross-reactivity with mouse and rat allows for use in rodent model studies; verify species-specific reactivity when using non-human tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to high-quality IVD raw materials, including validated monoclonal antibodies like this RanBP9 clone. Our product portfolio supports studies on ubiquitination, cell signaling, and cancer biology, with bulk supply options and custom sourcing available. From discovery to clinical application, CamelBio streamlines your workflow with reliable reagents and technical expertise.