Anti-RANBP2 Polyclonal Antibody for WB, IHC-P, ELISA - P49792

Core Product Specifications and Parameters

Biological Background: RANBP2 Function and Localization

• RANBP2, also known as NUP358, is a 358 kDa nucleoporin and a major structural component of the nuclear pore complex, localizing to the nucleus, nuclear envelope, and NPC.
• Acts as an E3 SUMO-protein ligase that facilitates SUMO1 and SUMO2 conjugation by interacting with UBE2I. Related references: PMID:11792325, PMID:12032081, PMID:15378033
• Contains F-G repeat domains that serve as docking sites for Ran-GTP and karyopherins, mediating nuclear protein import. Related references: PMID:7775481
• Binds single-stranded RNA in vitro and may also bind DNA. Related references: PMID:7775481
• Functions as a specific docking site for the nuclear export factor exportin-1 and is a component of the nuclear export pathway; also inhibits EIF4E-dependent mRNA export. Related references: PMID:10078529, PMID:22902403
• Sumoylates PML at Lys-490, which is critical for the proper assembly of PML nuclear bodies. Related references: PMID:22155184
• Recruits BICD2 to the nuclear envelope and to annulate lamellae during the G2 phase of the cell cycle. Related references: PMID:20386726
• Contains a PPIase-like domain but lacks peptidyl-prolyl cis-trans isomerase activity. Related references: PMID:20676357, PMID:23353830

Experimental Guidance and Technical Tips

• Consider using a low‑percentage SDS‑PAGE gel (e.g., 6%) and extended wet transfer times for improved resolution and transfer of the high‑molecular‑weight RANBP2 protein (~358 kDa).
• Validate the antibody in your relevant sample system, as expression levels and post‑translational modifications may vary across tissues and conditions.
• For IHC‑P, optimal antigen retrieval and antibody dilution should be empirically determined; heat‑mediated citrate buffer (pH 6.0) is a common starting point for nuclear antigens.
• The immunogen corresponds to a C‑terminal region (amino acids 2900–3050), which may recognize full‑length RANBP2 as well as potential isoforms or cleavage products.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with a one‑stop supply chain for IVD raw materials. We provide validated antibody pairs, optimized polyclonal antibodies like this anti‑RANBP2 rabbit pAb, and bulk ancillary reagents to accelerate your nucleocytoplasmic transport and SUMOylation studies.