Anti-RANBP10 Rabbit Polyclonal Antibody for WB and ELISA - Q6VN20

Core Product Specifications and Parameters

Biological Background: RANBP10 Function and Localization

• Acts as a core component of the CTLH E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Related references: PMID:29911972
• Enhances dihydrotestosterone-induced transactivation of the androgen receptor (AR) and dexamethasone-induced transactivation of the glucocorticoid receptor (NR3C1), but does not affect estrogen-induced transactivation. Related references: PMID:18222118
• Functions as a guanine nucleotide exchange factor (GEF) for RAN GTPase, potentially regulating nucleocytoplasmic transport and mitotic spindle assembly.
• May play an essential role in hemostasis and in maintaining microtubule dynamics with respect to platelet shape and function.
• Localizes to the cytoplasm, cytosol, and nucleus, supporting its roles in ubiquitination and intracellular signaling.
• Broadly expressed across tissues, with highest levels in skeletal muscle.
• Post-translational modifications include acetylation and phosphorylation; undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 362–497, so the antibody is expected to detect full-length RANBP10 (~67 kDa) and may recognize isoforms containing this region.
• For Western blot, consider using whole‑cell lysates from human or mouse cell lines. A band near 67 kDa is expected; specificity can be confirmed by siRNA‑mediated knockdown or overexpression.
• For ELISA, the polyclonal antibody can be employed as a capture or detection reagent; optimal pairing and dilution should be determined empirically.
• As with any polyclonal antibody, lot‑to‑lot variability is possible; titrate the antibody for each new application and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one‑stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and researchers from concept to clinic. This RANBP10 antibody complements research into ubiquitin‑proteasome pathways, hormone receptor signaling, and platelet biology. Drawing on a broad portfolio, CamelBio can supply validated antibody pairs, recombinant proteins, and bulk ancillary reagents to accelerate your assay development.