Anti-RANBP1 Polyclonal Antibody for WB, ELISA - P43487

Core Product Specifications and Parameters

Biological Background: RANBP1 Function and Localization

• RANBP1, also known as Ran-binding protein 1 or HTF9A, functions as a Ran-specific GTPase-activating protein, playing a pivotal role in the Ran GTPase cycle. Related references: PMID:7882974
• It inhibits the RCC1-dependent exchange of Ran-bound GDP by GTP, maintaining Ran in a GDP-bound state. Related references: PMID:7616957, PMID:7882974
• RANBP1 promotes GTP hydrolysis by enhancing RANGAP1 activity, thereby facilitating the conversion of RanGTP to RanGDP. Related references: PMID:7882974
• It mediates the dissociation of Ran from nuclear transport receptors, such as XPO1, triggering cargo release during nucleocytoplasmic transport. Related references: PMID:20485264
• Essential for mitotic spindle assembly and progression through mitosis, RANBP1 regulates Ran gradients critical for cell division. Related references: PMID:17671426
• The protein undergoes alternative splicing, producing multiple isoforms, and is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• For Western Blot, a target band at approximately 23 kDa is expected. Validate in relevant cell or tissue lysates from human, mouse, or rat.
• Optimize antibody dilutions and blocking conditions empirically, as reactivity may vary with sample preparation and detection systems.
• For ELISA, consider using the recombinant protein or peptide as coating antigen to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. Our portfolio includes validated antibodies for nucleocytoplasmic transport and mitosis research, alongside bulk ancillary reagents and custom sourcing for rare targets. With RANBP1 antibodies, we help streamline your assay development and supply chain.