Anti-Ran Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62826

Core Product Specifications and Parameters

Biological Background: Ran Function and Localization

• Ran (TC4, Gsp1, ARA24) is a small GTPase of the Ras superfamily that functions as a molecular switch in nucleocytoplasmic transport. Related references: PMID:10400640, PMID:17209048, PMID:26272610
• It cycles between an inactive GDP-bound cytoplasmic form and an active GTP-bound nuclear form, regulating cargo loading and release by importin/exportin receptors. Related references: PMID:11336674, PMID:26272610, PMID:29040603
• Ran-GTP binds directly to import receptors, causing release of cargo into the nucleus, while export receptors require Ran-GTP for cargo binding. Related references: PMID:8896452, PMID:9351834, PMID:9428644
• Interaction with RANBP1 induces a conformational change in the XPO1-Ran complex, triggering nuclear export signal release. Related references: PMID:20485264
• During mitosis, Ran-GTP triggers microtubule assembly at chromosomes and is essential for spindle formation and chromosome segregation. Related references: PMID:10408446, PMID:29040603, PMID:12194828, PMID:8421051
• The complex with BIRC5/survivin scaffolds the effector TPX2 to microtubules, facilitating spindle assembly. Related references: PMID:18591255
• Ran also negatively regulates VRK1 and VRK2 kinases and enhances androgen receptor (AR)-mediated transactivation, with sensitivity to AR poly-Gln length. Related references: PMID:18617507, PMID:10400640
• Subcellular localization includes nucleus, nuclear envelope, cytosol, and melanosomes; post-translational modifications include acetylation, phosphorylation, and ubiquitination.
• Widely expressed across tissues (Human Protein Atlas).

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human Ran protein (aa 1–216). For Western blot (WB), the predicted molecular weight is 24 kDa; validate detection in whole-cell lysates of human, mouse, or rat cell lines.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilization is required to detect nuclear and nuclear envelope pools. Use appropriate positive control cells known to express Ran.
• The antibody has been tested for ELISA; titer and working dilutions should be optimized in your specific assay system.
• Upon arrival, store at –20°C. Avoid repeated freeze-thaw cycles to preserve activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials. This anti-Ran polyclonal antibody serves the nucleocytoplasmic transport and cell cycle research areas, and we can also supply validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents tailored to your development needs. For rare-target sourcing or custom antibody production, our team bridges gaps from concept to clinic, helping accelerate your assay development.