Anti-Ran Rabbit Monoclonal Antibody for WB, ELISA - P62826

Core Product Specifications and Parameters

Biological Background: Ran Function and Localization

• Ran (TC4, Gsp1, ARA24) is a small GTPase belonging to the Ras superfamily, encoded by the RAN gene in humans.
• Functions as a key regulator of nucleocytoplasmic transport, controlling the import and export of proteins and RNAs by cycling between GDP- and GTP-bound states; this cycling ensures directional cargo loading and release by transport receptors. Related references: PMID:10400640, PMID:17209048, PMID:26272610, PMID:11336674, PMID:29040603
• Essential for mitosis: in its GTP-bound form, Ran triggers microtubule assembly at mitotic chromosomes and is required for normal spindle formation and chromosome segregation; interacts with BIRC5/survivin to deliver TPX2 to microtubules. Related references: PMID:10408446, PMID:29040603, PMID:12194828, PMID:8421051, PMID:18591255
• Acts as a negative regulator of VRK1 and VRK2 kinase activity, and enhances AR-mediated transactivation in a polyglutamine length-dependent manner. Related references: PMID:18617507, PMID:10400640
• Subcellular localization includes the nucleus, nuclear envelope, cytoplasm, and melanosomes.
• Post-translationally modified by acetylation, phosphorylation, and isopeptide bond formation; sumoylation and ubiquitination have been detected.
• Widely expressed across human tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from residues 117–216 of human Ran; confirm specificity by peptide competition or knockdown validation.
• For WB, the predicted molecular weight is ~24 kDa; consider using a reducing SDS-PAGE protocol and a blocking buffer optimized for rabbit monoclonal antibodies.
• The antibody is suitable for ELISA; titrate starting from 1:1000 and validate with recombinant Ran protein or cell lysates.
• Cross-reactivity with mouse and rat Ran is reported; verify endogenous expression levels in your model system.
• Store as recommended upon receipt (shipment on ice bags); avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive pipeline of IVD raw materials, including validated monoclonal antibodies like this anti-Ran antibody, antibody pairs, and ancillary reagents. From target discovery to clinical assay development, CamelBio supports your Ran-related projects in oncology, cell cycle, and nucleocytoplasmic transport research with bulk supply and customized sourcing solutions. Partner with CamelBio to streamline your raw material procurement, leveraging our expertise in antibody development and rare-target sourcing.