Anti-RAMP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60895

Core Product Specifications and Parameters

Biological Background: RAMP2 Function and Localization

• RAMP2 functions as an accessory protein that interacts with and modulates the activity of G protein-coupled receptors such as calcitonin gene-related peptide type 1 receptor (CALCRL) and calcitonin receptor (CALCR), and is required for the transport of CALCRL to the plasma membrane. Related references: PMID:9620797
• Together with CALCRL, RAMP2 forms a receptor complex for adrenomedullin. Related references: PMID:22102369, PMID:32296767, PMID:9620797
• Together with CALCR, RAMP2 acts as a receptor complex for calcitonin. Related references: PMID:35324283
• Together with CALCR, RAMP2 also acts as a receptor complex for amylin. Related references: PMID:35324283
• RAMP2 localizes predominantly to the cell membrane.
• It is strongly expressed in lung, breast, immune system, and fetal tissues.
• Structural features include a signal peptide, a transmembrane helix, and disulfide bonds; the protein is glycosylated and alternative splicing produces multiple isoforms.
• The unprocessed precursor has a predicted molecular weight of approximately 19.6 kDa, consistent with the mature protein observed at about 20 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), validate the specific band at ~20 kDa in RAMP2-expressing tissues such as lung or breast; run appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), consider heat-induced antigen retrieval and test on known positive tissues (e.g., lung) to confirm staining pattern; include an isotype control.
• When using in ELISA, pair with a carefully selected detection antibody and validate performance using recombinant RAMP2 protein or lysates from transfected cells.
• The antibody cross-reacts with mouse and rat RAMP2; therefore, it can be applied in rodent models, but always verify specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for high-quality IVD raw materials, offering this anti-RAMP2 polyclonal antibody to support studies on adrenomedullin signaling and receptor pharmacology. Beyond individual antibodies, CamelBio provides validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—all backed by technical and regulatory guidance. Accelerate your diagnostic development with CamelBio, from concept to clinical application.