Anti-RALGPS1 Rabbit Polyclonal Antibody for WB and ELISA - Q5JS13

Core Product Specifications and Parameters

Biological Background: RALGPS1 Function and Localization

• Encodes a guanine nucleotide exchange factor (GEF) that specifically activates the small GTPase RALA by catalyzing GDP/GTP exchange, thereby regulating downstream signaling cascades.
• Also known as RalGEF2 and RalGPS1A; the protein contains a PH domain and an SH3-binding motif, which are characteristic of RalGEF family members and facilitate membrane targeting and protein-protein interactions.
• The RALGPS1 gene undergoes alternative splicing, producing at least two major isoforms with distinct tissue distribution patterns.
• Isoform 1 is prominently expressed in heart and testis, with lower levels in brain, pancreas, skeletal muscle, small intestine, and thymus; isoform 2 is more widely expressed in brain, colon, kidney, pancreas, prostate, skeletal muscle, small intestine, testis, thymus, and uterus.
• Subcellular localization studies show RALGPS1 present in both the cytoplasm and at the cell membrane, consistent with its role in spatial regulation of RALA signaling.
• Putatively involved in cytoskeletal organization, suggesting a function in cell shape, motility, or intracellular trafficking (by similarity).
• Structural studies (3D-structure) have provided insights into the catalytic mechanism of the Sec7 domain, underscoring its importance as a guanine-nucleotide releasing factor.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–300 of human RALGPS1 (NP_055451.1), a region that may be conserved across isoforms; thus, the antibody may detect multiple splice variants. Consider peptide blocking or isoform-specific controls to confirm specificity.
• For western blot, endogenous RALGPS1 migrates at approximately 62 kDa. Ensure thorough cell lysis and consider membrane protein enrichment protocols, as the protein associates with the plasma membrane.
• When performing ELISA, use a standard curve generated with the recombinant immunogen fragment for quantitative estimation, and validate the assay using human cell or tissue lysates.
• Given the wide tissue expression, validate the antibody in a panel of human tissues (e.g., heart, testis, brain) to confirm the expected band pattern.
• Since RALGPS1 is a GEF, co‑immunoprecipitation experiments can be coupled with WB to study its interaction with RALA; however, empirical optimization of lysis buffers is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities by providing ready access to IVD raw materials like this anti‑RALGPS1 polyclonal antibody, essential for investigating RALA‑mediated signaling in cancer and cell biology. Our platform streamlines procurement of validated antibody pairs, bulk ancillary reagents, and hard‑to‑find proteins, ensuring a reliable supply chain from discovery to assay development. Whether you need optimized monoclonal/polyclonal antibodies, recombinant antigens, or technical consulting, CamelBio serves as a single‑source bridge to accelerate your project’s transition from concept to clinic.