Anti-RALGDS Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q12967

Core Product Specifications and Parameters

Biological Background: RALGDS Function and Localization

• RALGDS (Ral guanine nucleotide dissociation stimulator), also known as RalGEF or RGF, is a guanine nucleotide exchange factor that specifically activates RalA and RalB small GTPases.
• It functions as a key effector downstream of Ras family proteins (H-Ras, K-Ras, R-Ras) and Rap, linking these signaling pathways to Ral-mediated intracellular transport.
• In innate immunity, RALGDS recognizes Lys-33-linked polyubiquitinated TRAF3 and facilitates exocyst complex assembly, contributing to bacterial clearance. Related references: PMID:27438768
• Subcellular localization includes both the cytoplasm and nucleus, suggesting dynamic shuttling and multiple functional compartments.
• Alternative splicing generates multiple isoforms, which may have distinct regulatory roles.
• Post-translational modifications include phosphorylation, as indicated by the phosphoprotein keyword, which could modulate its GEF activity.
• The protein is highly conserved and belongs to the reference proteome, underscoring its fundamental biological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 771–902) of human RALGDS. Consider verifying reactivity against your target species and isoform of interest, as this region may be conserved across human, mouse, and rat.
• For western blotting, a band near 100–101 kDa is expected under reducing conditions. Optimizing blocking and antibody dilution may be required for clear signal.
• In IHC-P, appropriate antigen retrieval and tissue fixation methods should be optimized; consider testing known positive tissues expressing RALGDS.
• For IF/ICC, verify subcellular localization (cytoplasmic and nuclear) with appropriate controls, as staining may vary depending on cell type and fixation.
• ELISA can be used for quantitative detection; pairing with a validated capture/detection antibody system is recommended for robust assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers investigating Ras/Ral signaling, intracellular transport, or innate immunity, our one-stop sourcing platform simplifies procurement from discovery to assay development. We also support rare and niche target raw-material sourcing to accelerate your diagnostic projects.