Anti-RALBP1 Rabbit Polyclonal Antibody (KO Validated) for WB and ELISA - Q15311

Core Product Specifications and Parameters

Biological Background: RALBP1 Function and Localization

• RALBP1 (RalA-binding protein 1), also known as RLIP76 or RIP1, is a multifunctional 76 kDa protein that acts as a downstream effector of the small GTPases RALA and RALB. Related references: PMID:7673236
• As a GTPase-activating protein (GAP), it inactivates CDC42 and RAC1 by stimulating their GTP hydrolysis, thereby modulating cytoskeletal dynamics and signaling. Related references: PMID:7673236
• RALBP1 participates in ligand-dependent endocytosis of EGF and insulin receptors; during mitosis, it scaffolds cyclin B-CDK1-mediated phosphorylation of EPSIN to halt endocytosis, ensuring faithful cell division. Related references: PMID:10910768, PMID:12775724
• At mitochondria, recruited by RALA, RALBP1 scaffolds cyclin B-CDK1 to phosphorylate and activate DNM1L, controlling mitochondrial fission during mitosis. Related references: PMID:21822277
• Additionally, RALBP1 functions as a primary ATP-dependent transporter for glutathione conjugates and mediates efflux of xenobiotics like doxorubicin, contributing to multidrug resistance.
• The protein localizes to the cell membrane, cytoplasm (including cytoskeleton and spindle poles), nucleus, and mitochondria, underscoring its multifaceted roles.
• Ubiquitously expressed at low levels, RALBP1 exhibits notably strong expression in erythrocytes.
• Post-translational modifications include acetylation and phosphorylation; the protein also contains ATP-binding and nucleotide-binding domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 396–655); verify that the antibody recognizes full‑length RALBP1 and any relevant cleavage products in your experimental system.
• For Western blot, use human and mouse cell lysates (e.g., HeLa, NIH/3T3) as positive controls, given the confirmed cross‑reactivity.
• In ELISA, consider using the recombinant fusion protein employed as immunogen as a coating antigen or standard, and optimize antibody dilutions accordingly.
• As a knockout‑validated antibody, confirm specificity by comparing signals between wild‑type and RALBP1‑knockout samples when available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive IVD raw material solutions, including this RALBP1 polyclonal antibody for studies on Ral signaling, endocytosis, mitochondrial dynamics, and drug resistance. Our portfolio features validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom rare‑target sourcing to support your assay development from concept to clinic. With one‑stop access to raw materials and technical services, CamelBio accelerates your path to reliable diagnostic products.