Anti-RALBP1 Monoclonal Antibody for IHC-P, IF/ICC, ELISA - Q15311

Core Product Specifications and Parameters

Biological Background: RALBP1 Function and Localization

• RALBP1, also known as RLIP76 or Ral-interacting protein 1, is a ~76 kDa multifunctional protein encoded by the RALBP1 gene (GeneID: 10928).
• Acts as a downstream effector of RALA and RALB, and functions as a GTPase-activating protein (GAP) that inactivates CDC42 and RAC1 by stimulating their intrinsic GTPase activity. Related references: PMID:7673236
• Participates in Ral signaling-dependent regulation of receptor-mediated endocytosis, including ligand-dependent internalization of EGF and insulin receptors. Related references: PMID:10910768, PMID:12775724
• During mitosis, serves as a scaffold for cyclin B-CDK1-mediated phosphorylation of EPSIN/EPN1, inhibiting endocytosis; also facilitates mitochondrial fission by scaffolding DNM1L phosphorylation at mitochondria. Related references: PMID:12775724, PMID:21822277
• Possesses ATP-dependent transport activity for glutathione conjugates and xenobiotics like doxorubicin, potentially contributing to multidrug resistance in cancer cells.
• Subcellularly localizes to multiple compartments including the cell membrane, cytosol, cytoskeleton (spindle pole), nucleus, and mitochondrion.
• Ubiquitously expressed at low levels, with notably strong expression in erythrocytes.
• Post-translationally modified by acetylation and phosphorylation; classified under keywords such as ATP-binding, GTPase activation, and translocase.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200–300; consider that the epitope may be partially buried in native conformation, potentially requiring denaturation or appropriate antigen retrieval for immunohistochemistry.
• For IHC-P, standard heat-induced epitope retrieval (e.g., citrate buffer pH 6.0) and optimized primary antibody concentration are recommended; validate in species with confirmed cross-reactivity (human, mouse, rat).
• In IF/ICC, permeabilization with 0.1–0.3% Triton X-100 may improve nuclear or mitochondrial antigen accessibility; include appropriate negative controls.
• ELISA applications benefit from titrating the antibody against the immunogen peptide or recombinant RALBP1 protein to establish optimal coating concentration and detection range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored to diagnostic development. Our one-stop platform supports research areas including oncology and drug resistance, enabling seamless scaling from concept to clinic. For RALBP1-targeted projects, we facilitate access to rare-target raw materials and customized supply solutions, helping partners accelerate assay performance and manufacturing readiness.