Anti-RALB Polyclonal Antibody for WB, ELISA - P11234

Core Product Specifications and Parameters

Biological Background: RALB Function and Localization

• RALB encodes Ras-related protein Ral-B, a small GTPase that cycles between active GTP-bound and inactive GDP-bound conformations.
• Functions as a multifunctional GTPase regulating gene expression, cell migration, proliferation, oncogenic transformation, and membrane trafficking. Related references: PMID:10393179 PMID:17875936 PMID:18756269
• Serves as a GTP sensor for dense core vesicle exocytosis, stabilizing the exocyst complex and targeting it to the leading edge of migrating cells (by similarity).
• Required for suppression of apoptosis, promoting cell survival through downstream effector interactions. Related references: PMID:17875936
• During late cytokinesis, recruits the exocyst to the midbody to drive abscission, the final step of cell division. Related references: PMID:18756269
• Involved in ligand-dependent receptor-mediated endocytosis of EGF and insulin receptors, linking Ral-B to growth factor signaling. Related references: PMID:10393179
• Localizes primarily at the cell membrane and transiently at the midbody during cytokinesis.
• Post-translationally modified by prenylation for membrane anchorage and methylation; classified under keywords such as GTP-binding, hydrolase, lipoprotein, and alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 23 kDa is expected; use whole-cell lysates from human, mouse, or rat cell lines as positive controls.
• Polyclonal antibodies may exhibit lot-to-lot variability; validate each new lot using a known positive control to ensure consistent performance.
• For ELISA, optimize coating antigen concentration and detection conditions; the recombinant immunogen can serve as a positive control.
• Consider confirming specificity by siRNA knockdown or overexpression experiments in relevant cell models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This RALB polyclonal antibody supports research into GTPase signaling pathways, exocytosis, apoptosis, and cell migration. Beyond this product, we offer bulk procurement options for validated antibody pairs, optimized monoclonal and polyclonal antibodies, ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development.