Anti-RAIDD/CRADD Rabbit Polyclonal Antibody for WB, ELISA - P78560

Core Product Specifications and Parameters

Biological Background: RAIDD/CRADD Function and Localization

• CRADD (also known as RAIDD) is a death domain-containing adapter protein encoded by the CRADD gene (Gene ID: 8738).
• It forms the PIDDosome complex with PIDD1 and caspase-2 (CASP2), activating CASP2 to trigger apoptosis. Related references: PMID:15073321 PMID:16652156 PMID:17159900
• CRADD also recruits CASP2 to the TNFR-1 signaling complex via interactions with RIPK1 and TRADD, suggesting a role in TNF-mediated signaling. Related references: PMID:8985253
• The protein localizes to both the cytoplasm and nucleus.
• CRADD is constitutively expressed across tissues, with highest levels in adult heart, testis, liver, skeletal muscle, and fetal liver and kidney.
• Alternative splicing yields multiple isoforms, and mutations in CRADD are associated with intellectual disability and lissencephaly.
• It is classified under keywords including apoptosis, alternative splicing, disease variant, and 3D-structure, highlighting its functional and clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–199 of human RAIDD/CRADD (NP_003796.1); expect reactivity against the N-terminal region.
• For western blotting, a band around 23 kDa is predicted. Validate using human or mouse cell lysates known to express CRADD.
• In ELISA applications, the polyclonal nature may provide enhanced sensitivity; however, assay conditions must be optimized for each experimental system.
• Consider testing cross-reactivity with other species if required, as the antibody is reported to recognize human and mouse proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to supply high-quality IVD raw materials like this anti-RAIDD/CRADD polyclonal antibody, which targets a key apoptosis regulator. Whether you need validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, our one-stop platform bridges the gap from concept to clinic. Contact us to discuss how we can support your assay development workflow.