Anti-RAIDD/CRADD Rabbit Monoclonal Antibody for WB, ELISA - P78560

Core Product Specifications and Parameters

Biological Background: RAIDD/CRADD Function and Localization

• Protein Identity and Isoforms: CRADD (Death domain-containing protein CRADD), also known as RAIDD, is a member of the death domain superfamily. Alternative splicing gives rise to multiple isoforms.
• Core Function in PIDDosome and Apoptosis: Serves as an adapter protein in the PIDDosome complex, interacting with PIDD1 and CASP2 to activate CASP2 and trigger apoptosis.
  Related references: PMID:15073321 | PMID:16652156 | PMID:17159900
• Role in TNFR-1 Signaling: Recruits CASP2 to the TNFR-1 signaling complex through interactions with RIPK1 and TRADD, potentially mediating tumor necrosis factor-induced signaling.
  Related references: PMID:8985253
• Subcellular Localization: Localizes to both the cytoplasm and nucleus.
• Tissue Expression: Constitutively expressed across human tissues, with notably high levels in adult heart, testis, liver, skeletal muscle, and fetal liver and kidney.
• Clinical Relevance: Mutations in CRADD are associated with intellectual disability and lissencephaly; the protein is a proteomics identification target and its 3D structure has been determined.

Experimental Guidance and Technical Tips

• For Western blotting, a predominant band at approximately 23 kDa is expected; use lysates from high-expressing tissues such as human heart or testis to maximize signal.
• The antibody is validated for ELISA; consider its performance in direct or sandwich formats with appropriate controls.
• The immunogen is a synthetic peptide spanning amino acids 100–199, so binding to native full-length protein should be confirmed in the intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable one-stop channel for IVD raw materials. This anti-RAIDD/CRADD antibody exemplifies our capability to supply high-quality monoclonal antibodies for apoptosis-related biomarkers. We also offer validated antibody pairs, optimized reagents, and bulk sourcing for ancillary materials to accelerate your assay development from concept to clinic.