Anti-RAI14 Rabbit Polyclonal Antibody for WB and ELISA - Q9P0K7

Core Product Specifications and Parameters

Biological Background: Ankycorbin Function and Localization

• Ankycorbin (RAI14, also known as RAI13 and NORPEG) is a 110 kDa protein characterized by ankyrin repeats and coiled-coil domains, cataloged in the UniProt reference proteome (Q9P0K7).
• It plays a critical role in actin regulation at the ectoplasmic specialization, a testis-specific cell junction essential for establishing sperm polarity and normal spermatid adhesion.
• RAI14 is implicated in maintaining Sertoli cell tight junction integrity at the blood-testis barrier, thereby supporting spermatogenesis.
• The protein localizes to the cytoplasm, cytoskeleton (including stress fibers and cell cortex), cell junctions, and nucleus.
• Tissue expression is highest in placenta, muscle, kidney, and testis, with moderate levels in heart, brain, lung, liver, and intestine; isoform 2 is detected in fetal and adult testes and spermatozoa.
• Post-translational modifications include acetylation and phosphorylation, and alternative splicing yields isoforms with roles in differentiation and spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–600 of human RAI14; consider using the recombinant protein as a positive control in ELISA and as a blocking agent if needed.
• For Western blotting, validate the antibody in lysates from human, mouse, or rat testis or other high-expression tissues, and confirm the expected band near 110 kDa.
• When using ELISA, titrate the antibody against the immunogen or full-length protein to determine optimal working concentrations.
• The antibody cross-reacts with mouse and rat RAI14, but epitope conservation should be verified if absolute specificity is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner, offering a complete portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing services. This anti‑RAI14 polyclonal antibody exemplifies our capability to supply high‑quality reagents for reproductive biology and cytoskeleton research. From concept to clinic, we support diagnostic manufacturers and laboratories with integrated technical services and reliable supply chains.