Anti-RAG2 Rabbit Polyclonal Antibody for WB, ELISA - P55895

Core Product Specifications and Parameters

Biological Background: V(D)J Recombination-Activating Protein 2 Function and Localization

• V(D)J recombination-activating protein 2 (RAG2) is a core component of the RAG complex, essential for assembling diverse immunoglobulin and T-cell receptor genes during lymphocyte development.
• RAG2 is required for all catalytic activities of RAG1, mediating DNA cleavage via nicking and hairpin formation to generate coding and signal ends.
• RAG2 acts as a chromatin state sensor, binding histone H3 trimethylated at Lys4 (H3K4me3) to stimulate both nicking and hairpin steps.
• The RAG complex contributes to pre-B cell allelic exclusion by inducing ATM-dependent repositioning of the unrecombined allele to pericentromeric heterochromatin.
• RAG2 localizes to the nucleus and its expression is restricted to cells of the B- and T-lymphocyte lineages.
• UniProt keywords highlight its roles in chromatin regulation, DNA recombination, and disease association with severe combined immunodeficiency (SCID).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 258-527, a region including the C-terminal PHD finger domain critical for H3K4me3 recognition; this may yield antibodies detecting full-length and some truncated isoforms.
• For western blot (WB), consider optimizing the dilution (commonly 1:500–1:2000 for polyclonal antibodies) and include appropriate lymphocyte lineage cell lysates as positive controls.
• In ELISA applications, use recombinant RAG2 protein or peptide as coating antigen; cross-reactivity with mouse RAG2 enables validation in murine systems.
• Due to the polyclonal nature, confirm lot-to-lot consistency and validate in the specific sample system (e.g., primary lymphocytes, thymocytes) to ensure expected performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams a streamlined pathway to IVD raw materials, from concept to clinic. This anti-RAG2 antibody supports lymphocyte development and V(D)J recombination research, and we can source validated antibody pairs, bulk ancillary reagents, or rare target materials to complete your assay development needs.