Anti-RAE1 Rabbit Polyclonal Antibody for WB, ELISA - P78406

Core Product Specifications and Parameters

Biological Background: RAE1 Function and Localization

• RAE1, also known as Gle2, MRNP41, or mRNA export factor RAE1, is a 41kDa WD repeat-containing protein encoded by the RAE1 gene (Gene ID: 8480).
• RAE1 functions as a key mRNA export factor, facilitating nucleocytoplasmic transport of messenger ribonucleoproteins (mRNPs) (PubMed:20498086, PubMed:33849972). Related references: PMID:20498086, PMID:33849972
• It plays a critical role in mitotic bipolar spindle formation, ensuring proper cell division (PubMed:17172455). Related references: PMID:17172455
• RAE1 may also link cytoplasmic mRNPs to the cytoskeleton, potentially regulating mRNA localization and translation (PubMed:17172455). Related references: PMID:17172455
• Subcellularly, RAE1 localizes to the cytoplasm, nucleus, nuclear envelope, and spindle poles, consistent with its dual functions in transport and mitosis.
• Post-translational modifications, including phosphorylation, may regulate its activity, and it is listed as a phosphoprotein in proteomics databases.
• Keywords associated with RAE1 include host-virus interaction, suggesting involvement in viral RNA export or replication.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-368 of human RAE1 (NP_003601.1, full-length), raising antibodies that likely recognize both endogenous and recombinant protein forms.
• For Western blot (WB), anticipate a band near 41kDa; consider using appropriate blocking and washing conditions to minimize background, as polyclonal antibodies may exhibit non-specific binding.
• Cross-reactivity with mouse and rat RAE1 is reported; validate performance in the relevant species model as sequence homology may influence recognition.
• For ELISA, coating with recombinant RAE1 or peptide fragments may be optimized; antibody titer and specificity should be experimentally determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined sourcing solution for critical IVD raw materials like the RAE1 Rabbit pAb. In the field of mRNA transport and cell division research, our validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents support robust assay development. Leverage our one-stop supply chain for target-specific raw materials, from concept to clinic, ensuring consistent quality and performance for your diagnostic and research applications.