Anti-Radixin Rabbit Monoclonal Antibody for WB, ELISA - P35241

Core Product Specifications and Parameters

Biological Background: Radixin Function and Localization

• Radixin is an actin-binding protein likely crucial for linking barbed ends of actin filaments to the plasma membrane.
• It localizes to the cell membrane, cytoplasm, cytoskeleton, cleavage furrow, microvilli, and stereocilia.
• Keywords include actin capping, actin-binding, cytoskeleton, membrane, and cell projection.
• Mutations in RDX are associated with non-syndromic deafness, autosomal recessive 24 (DFNB24).
• Radixin undergoes phosphorylation and is classified as a phosphoprotein.
• Alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 350-450 of human Radixin; for WB, validate the antibody in systems expressing the target protein endogenously or via overexpression.
• Predicted molecular weight is ~69 kDa; consider appropriate gel percentage and transfer conditions.
• Cross-reactivity with human, mouse, and rat is reported; verify in the relevant species' sample lysates.
• For ELISA, use the immunogenic peptide or recombinant Radixin protein as coating antigen; optimize coating buffer and antibody dilutions.

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CamelBio provides one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-Radixin antibody, and bulk ancillary reagents for diagnostic development. We support your research in cytoskeleton dynamics and hearing loss diagnostics, ensuring seamless supply from concept to clinic.