Anti-Radixin Polyclonal Antibody for WB and ELISA - P35241

Core Product Specifications and Parameters

Biological Background: Radixin Function and Localization

• Radixin is encoded by the RDX gene (also known as DFNB24) and belongs to the ezrin-radixin-moesin (ERM) family of proteins that link actin filaments to the plasma membrane.
• It plays a crucial role in binding the barbed end of actin filaments to the plasma membrane, thereby regulating cell shape, motility, and membrane organization.
• Radixin is localized to the cell membrane, cytoplasm, cytoskeleton, cleavage furrow, and specialized cell projections such as microvilli and stereocilia.
• The protein is subject to alternative splicing, producing isoforms that may have distinct functional roles.
• Radixin is phosphorylated, a post-translational modification important for its activation and membrane-cytoskeleton crosslinking activity.
• Mutations in the RDX gene are associated with non-syndromic deafness (DFNB24), highlighting its essential role in the function of cochlear hair cell stereocilia.
• Keywords from UniProt include actin capping, actin-binding, and deafness, reflecting its dual roles in cytoskeletal dynamics and hearing.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to amino acids 1–200 of human Radixin (N-terminal region). This region contains the FERM domain, which is critical for membrane association.
• For Western blot (WB), the predicted molecular weight of human Radixin is ~69 kDa. Use whole cell lysates from human, mouse, or rat tissues/cells known to express Radixin (e.g., epithelial cells, lymphocytes) as positive controls.
• In ELISA, consider using recombinant Radixin protein or cell extracts as coating antigens. Optimize antibody dilution and blocking conditions empirically.
• Cross-reactivity with mouse and rat Radixin has been reported, but sequence homology may affect detection efficiency; validate in the relevant sample system if working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting to support your diagnostic and research programs from concept to clinic. For studies on actin cytoskeleton and membrane linkage involving Radixin, we provide validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities also extend to rare targets, enabling your assay development with reliable and customizable supply solutions.