Anti-RAD9A Polyclonal Antibody for WB, IHC-P, ELISA - Q99638

Core Product Specifications and Parameters

Biological Background: RAD9A Function and Localization

• RAD9A is a component of the 9-1-1 checkpoint complex that plays a major role in DNA repair. Related references: PMID:10713044 PMID:17575048 PMID:20545769
• The 9-1-1 complex is recruited to DNA lesions by RAD17-RFC and acts as a sliding clamp platform for long-patch base excision repair (LP-BER) proteins. Related references: PMID:21659603
• The complex stimulates DNA polymerase beta (POLB), flap endonuclease FEN1, and DNA ligase I (LIG1) activities on LP-BER substrates. Related references: PMID:21659603
• RAD9A has intrinsic 3'->5' double-stranded DNA exonuclease activity. Related references: PMID:10713044
• RAD9A localizes to the nucleus, consistent with its DNA repair functions.
• RAD9A is a phosphoprotein, indicating regulation by post-translational modification.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 43 kDa (predicted weight) may be observed; validate with positive control lysates from human or rat cells.
• For immunohistochemistry (IHC-P), antigen retrieval methods should be optimized, and appropriate tissue controls should be included.
• For ELISA, the antibody can be used as a capture or detection reagent; optimal dilutions should be determined empirically.
• As a polyclonal antibody, it recognizes multiple epitopes within the immunogen region (aa 162-391), which may provide robust signal but could require careful validation for specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated provider of IVD raw materials, CamelBio offers comprehensive support for your diagnostic and research needs, including validated antibodies like this anti-RAD9A polyclonal antibody for DNA repair pathway studies. We supply optimized monoclonal and polyclonal antibodies, matched antibody pairs, bulk ancillary reagents, and can source rare targets to accelerate your assay development from concept to clinic. Partner with CamelBio to streamline your raw-material procurement with reliable, one-stop access.