Anti-RAD54L2 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q9Y4B4

Core Product Specifications and Parameters

Biological Background: RAD54L2 Function and Localization

• RAD54L2, also known as ARIP4 or RAD54-like protein 2, is a member of the RAD54 family of helicases.
• It functions as a DNA-dependent ATPase and helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-specific manner.
• RAD54L2 may stimulate the release of paused RNA polymerase II near transcription start sites of AR-target genes and unwind associated R-loops (PubMed:39028815). Related references: PMID:39028815
• It may also promote double-strand break formation by TOP2B, contributing to transcriptional output (PubMed:39028815). Related references: PMID:39028815
• RAD54L2 localizes primarily to the nucleus and associates with chromosomes.
• The protein is expressed at elevated levels in prostatic adenocarcinoma, suggesting a role in prostate cancer biology.
• Post translational modifications indicated by UniProt keywords include phosphorylation, isopeptide bond formation, and ubiquitin‑like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1178–1467 of human RAD54L2; antibodies raised against this region may detect full‑length protein and any isoforms that contain this segment.
• For IHC‑P, antigen retrieval (e.g., heat‑induced epitope retrieval in citrate buffer pH 6.0) and titration are recommended to achieve specific nuclear/chromosomal staining.
• In IF/ICC, use permeabilization with Triton X‑100 or digitonin and include appropriate controls to confirm nuclear localization.
• For ELISA, the antibody can be considered as a capture or detection reagent after direct validation of binding to the target antigen.
• Cross‑reactivity is indicated for human and rat; when using tissues or cells from other species, sequence homology should be checked and reactivity confirmed empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing. This anti‑RAD54L2 antibody addresses research needs in androgen signaling, transcription regulation, and prostate cancer, and we are ready to support custom formulations, scale‑up, and integrated supply solutions.