Anti-RAD52 Polyclonal Antibody for WB, ELISA - P43351

Core Product Specifications and Parameters

Biological Background: RAD52 Function and Localization

• RAD52 is a DNA repair protein that plays a central role in homologous recombination by mediating the annealing of complementary single-stranded DNA (ssDNA).
• It stimulates the recombination activity of RAD51 recombinase, facilitating the homology search and strand invasion steps of double-strand break (DSB) repair.
• Encoded by the RAD52 gene in humans, this protein is a key member of the RAD52 epistasis group, which is evolutionarily conserved and essential for maintaining genomic stability.
• RAD52 localizes predominantly to the nucleus, where it forms discrete repair foci in response to DNA damage.
• The protein is subject to alternative splicing, producing multiple isoforms that may have distinct regulatory roles.
• RAD52 is a phosphoprotein; its phosphorylation status can modulate its function and interactions with other repair proteins.
• Additional keywords associated with RAD52 include DNA-binding, DNA recombination, and 3D-structure, reflecting its structural and functional characteristics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 125-418 of human Rad52, covering regions involved in DNA binding and oligomerization. This polyclonal antibody is therefore expected to recognize the native, full-length protein.
• For Western blot applications, nuclear extracts are recommended as starting material to enrich for RAD52. Validate the antibody in your cell line or tissue of interest, and consider using a positive control such as HeLa cells, which express RAD52.
• Cross-reactivity with mouse and rat is indicated, making this antibody suitable for studies in rodent models. However, performance in other species should be independently verified.
• In ELISA, ensure proper antigen coating and antibody titration. The polyclonal nature may provide high sensitivity but also potential for cross-reactivity; appropriate blocking and washing steps are critical.
• Given RAD52's role in DNA repair, this antibody can be utilized in research exploring homologous recombination, response to genotoxic stress, and cancer biology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD and research laboratories, offering high-quality raw materials including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. With this anti-RAD52 antibody, researchers gain access to a critical tool for investigating DNA repair and recombination pathways, and CamelBio's sourcing expertise ensures consistent supply for demanding diagnostic and research applications. Whether you need rare target reagents or scalable raw materials, CamelBio bridges the gap from discovery to clinical development.