Anti-Rad51D Rabbit Polyclonal Antibody for WB, ELISA - O75771

Core Product Specifications and Parameters

Biological Background: Rad51D Function and Localization

• RAD51D (DNA repair protein RAD51 homolog 4), also known as R51H3, TRAD, and RAD51L3, is a member of the RAD51 paralog family involved in homologous recombination repair (HRR).
• The protein binds single-stranded DNA (ssDNA) and possesses DNA-dependent ATPase activity, crucial for its role in DNA repair.
• RAD51D is a component of the BCDX2 complex (RAD51B-RAD51C-RAD51D-XRCC2), which acts downstream of BRCA2 and upstream of RAD51 in the BRCA1-BRCA2-dependent HR pathway.
• The BCDX2 complex binds to Holliday junctions and replication fork junctions, and stimulates Holliday junction resolution by BLM.
• RAD51D localizes to the nucleus, cytoplasm, centrosome, and telomeres, reflecting its multifaceted roles in genomic maintenance.
• It is involved in telomere maintenance, contributing to chromosomal stability.
• Tissue expression analysis shows RAD51D is expressed in colon, prostate, spleen, testis, ovary, thymus, and small intestine, with weak expression in leukocytes.
• Alternative splicing of RAD51D generates multiple isoforms, expanding its functional repertoire (Keywords: Alternative splicing).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human Rad51D protein (residues 1-328), which may provide broad reactivity to various isoforms and post-translational modifications.
• For WB, consider using a total cell or nuclear extract from human cell lines known to express Rad51D (e.g., HeLa, HEK293). Validate antibody specificity by comparing signal in untreated vs. DNA-damaging agent-treated cells, as Rad51D levels may increase upon DNA damage induction.
• For ELISA, the antibody can be used as a capture or detection reagent; titration and pairing with a matched detection antibody is recommended. Always include appropriate positive (e.g., recombinant Rad51D protein) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for diagnostic manufacturers and researchers, offering a comprehensive catalog of IVD raw materials including validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents. This Rad51D antibody supports DNA repair and homologous recombination studies, and CamelBio can assist in sourcing complementary reagents and rare-target antibodies to streamline your assay development. Contact us for customized bulk supply and technical consultation.