Anti-RAD51C Rabbit Polyclonal Antibody for WB, ELISA - O43502

Core Product Specifications and Parameters

Biological Background: RAD51C Function and Localization

• RAD51C (DNA repair protein RAD51 homolog 3, also known as R51H3, BROVCA3, RAD51L2) is a member of the RAD51 paralog family and functions as an essential component of the homologous recombination (HR) DNA repair pathway.
• It participates in the repair of DNA double-strand breaks by forming two distinct complexes, BCDX2 (with RAD51B, RAD51D, XRCC2) and CX3 (with XRCC3), which act at different stages of HR, including RAD51 recruitment, branch migration, and Holliday junction resolution.
• RAD51C contributes to DNA cross-link resistance, sister chromatid cohesion, and genomic stability; it protects RAD51 from ubiquitin-mediated degradation and regulates mitochondrial DNA copy number under oxidative stress.
• The protein localizes to the nucleus, cytoplasm, perinuclear region, and mitochondria, reflecting its roles in both nuclear DNA repair and mitochondrial maintenance.
• Expression is broad but highest in testis, heart muscle, spleen, and prostate, indicating tissue-specific relevance in DNA damage responses.
• RAD51C undergoes phosphorylation and contains ATP-binding and DNA-binding domains critical for its activity.
• Mutations in RAD51C are associated with Fanconi anemia and increased susceptibility to breast and ovarian cancers.
• Alternative splicing generates multiple isoforms, potentially modulating function in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-135) of human RAD51C; consider this epitope location when designing siRNA or knockout validation experiments.
• For WB, the predicted molecular weight is ~42 kDa; optimize loading controls and antibody dilutions in your specific sample type.
• Cross-reactivity with mouse suggests potential utility in murine models; validate performance in relevant mouse tissues or cell lines.
• In ELISA applications, ensure proper coating and detection conditions for the recombinant protein or peptide fragment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to premium IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This RAD51C antibody supports DNA damage and repair research, and our team can assist in sourcing rare-target reagents and providing technical consultation to accelerate your assay development pipeline. From concept to clinic, CamelBio bridges your sourcing needs with reliable, high-quality supplies.