Anti-RAD51C Polyclonal Antibody for IF/ICC and ELISA - O43502

Core Product Specifications and Parameters

Biological Background: RAD51C Function and Localization

• RAD51C, also known as DNA repair protein RAD51 homolog 3 or RAD51L2, is a member of the RAD51 paralog family involved in homologous recombination (HR) repair of DNA double-strand breaks.
• It is a core component of the BCDX2 (RAD51B-RAD51C-RAD51D-XRCC2) and CX3 (RAD51C-XRCC3) complexes, which act at distinct stages of the BRCA1-BRCA2-dependent HR pathway, from RAD51 recruitment to Holliday junction resolution.
• RAD51C plays an early role in DNA damage signaling by facilitating CHEK2 phosphorylation, leading to cell cycle arrest and HR activation, and also participates in late-stage branch migration and HJ resolution.
• Subcellular localization includes the nucleus, cytoplasm, perinuclear region, and mitochondrion, reflecting its multifaceted roles in genomic maintenance and mitochondrial DNA copy number regulation under oxidative stress.
• Post-translational modifications include phosphorylation, and it is classified under keywords such as DNA damage, DNA recombination, DNA repair, and Fanconi anemia; mutations in RAD51C are associated with Fanconi anemia complementation group O and breast cancer susceptibility.
• RAD51C is widely expressed, with highest levels in testis, heart muscle, spleen, and prostate, consistent with its critical function in meiotic recombination and proliferative tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-135) of human RAD51C; the antibody may recognize epitopes within this conserved domain and could be suitable for detecting both full-length and truncated isoforms.
• For IF/ICC applications, consider fixing cells with paraformaldehyde and permeabilizing with detergent to allow nuclear access, as RAD51C shuttles between cytoplasm and nucleus.
• In ELISA, the polyclonal nature may provide high sensitivity, but cross-absorption or blocking steps are recommended to minimize background from other RAD51 paralogs sharing sequence homology.
• Validate species cross-reactivity carefully; this antibody is raised against human RAD51C and cross-reactivity is listed for human only; performance in other species should be experimentally confirmed if needed.
• Given the ~42 kDa molecular weight, use appropriate prestained markers and confirm band identity with positive and negative cell lysates where RAD51C expression levels are known (e.g., testis or cancer cell lines).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets across diagnostic development stages. This RAD51C antibody supports HR deficiency and Fanconi anemia research, and CamelBio can complement it with recombinant proteins, matched detection antibodies, and technical consultation to accelerate your assay development. From concept to clinic, trust CamelBio as your reliable partner for high-quality immunoreagents and tailored technical support.